[Yeast Nitrogen Broth (without Amino Acids and Ammonium Sulfate)]

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Yeast Nitrogen Broth (without Amino Acids and Ammonium Sulfate)

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Yeast Nitrogen Broth (without Amino Acids and Ammonium Sulfate) [MB-Y0796]

Medium used for the isolation and cultivation of yeasts based on carbon and nitrogen assimilation.

PACKAGE

Description	Quantity	Cat. No
Yeast Nitrogen Broth (without Amino Acids and Ammonium Sulfate)	500g	MB-Y0796

PROCEDURE

Medium should be prepared in 10X strength. Suspend 1.7 G of powder in 100mL of distilled water or deionized water. If necessary, add 5 G of dextrose or equivalent amount of another carbohydrate, ammonium sulfate and desired amino acid. Mix well and sterilize the medium by filtration. DO NOT HEATING. Prepare the final medium by aseptically pipetting 1 mL of the solution into 9 mL of distilled or deionized water in tubes. Mix well. Keep refrigerated until used.

STORE

The powder is very hygroscopic. Store the powder at room temperature, in a dry environment, in its original container tightly closed and use it before the expiry date on the label. Store prepared medium at 2 - 8°C.