ALOA agar,MB-A1620 (식품공전 100번 배지)

* Listeria monocytogenes 를 배양하기 위한 배지입니다.

* Color Listeria [MB-L1615] 와 Color O.A. Listeria[MB-L1620] 가 ALOA Agar로 통합되었습니다.

* 식품공전 제2019-57호에 추가된 리스테리아 모노사이토제네스 선택배지입니다.

 

 

 

ALOA Agar (Agar Listeria) [MB-A1620]

                                                                                                                                                   

 

Medium used for the isolation and identification of Listeria monocytogenes.

* Equally use with MFDS (MB-A1620K) and Color O.A. Listeria Agar (MB-C1620).

 

 

PACKAGE

 

Description	Quantity	Cat. No
ALOA Agar	500g	MB-A1620
ALOA Agar	*500ml x 10	MB-A1620M
*O.A Listeria Agar supplement (필수첨가)	4ml / 냉동	MB-O0774
*Phosphatidyl Inositiol supplement (MB-L3072 중 택1)	25ml/ 냉장	MB-P0775
*Lecithin solution (MB-P0775 중 택1)	25ml/ 냉장	MB-L3072

* 500ml x 10 제품의 경우, 500ml 배지를 제조할 수 있는 파우더 양만큼 소분되어있는 제품입니다.

* 식품공전에 따라 실험하는 경우에는 O.A Listeria Agar supplement 와 Phosphatidyl Inositol supplement를 첨가해야합니다.

 

 

 

* O.A. Listeria Agar supplement (별도 구매 필요)

 

Contents	g
1 vial contents (each vial is sufficient for 500mL of medium)
Polymyxin B	38,350 IU
Ceftazidime	0.01 g
Nalidixic Acid	0.01 g
Cycloheximide	0.025 g

 

 

 

* Phosphatidyl Inositiol supplement (별도 구매 필요)

 

Contents	g
1 vial contents (each vial is sufficient for 500mL of medium)
L-α-phosphatidylinositol	1.0 g

 

 

 

* Lecithin solution (별도 구매 필요)

 

Contents	mL
1 vial contents (each vial is sufficient for 500mL of medium)
Lecithin solution	25mL

 

 

 

PROCEDURE

 

Suspend 72.05 G of powder in 942 mL of distilled or deionized water. Heat to boiling until completely dissolved. Sterilize by autoclave at 121°C for 15 minutes. Cool to 45 - 50°C in water bath. Aseptically add 2 vials of O.A. Listeria Agar supplement (MB-O0774). And add 2 vials of Phosphatidyl Inositol supplement (MB-P0775) or Lecithin Solution (MB-L3072) to observe phospholipase reaction. Mix well. Pour into petri dishes.

 

 

STORE

 

The powder is very hygroscopic. Store the powder at room temperature, in a dry environment, in its original container tightly closed and use it before the expiry date on the label. Store prepared medium at 2 - 8°C.