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Lugol PVP Solution (Gram staining) [MB-80296]
PACKAGE
Description |
Quantity |
Cat. No |
Lugol PVP
Solution (Gram staining) |
1000mL |
MB-80296 |
PROCEDURE (±×¶÷¿°»ö °úÁ¤¿¡ ´ëÇÑ ¼³¸íÀÔ´Ï´Ù)
Preparation
and fixing
On clean slides, make a smear of the culture or
pathological material. Leave to dry in the air and fix by heat, passing rapidly
over the flame. Do not overheat the sample when fixing. Other fixing methods
may be used.
Staining
1. Cover the slide with the Crystal Violet Solution. Wait
1 minute, then rinse gently with water.
2. Cover the slide with the Lugol PVP Solution. Wait 1
minute.
3. Decolor with the decolorizing Solution for as long as
the preparation releases color (about 30-60
seconds), then rinse delicately with water.
4. Cover the slide with the Safranine Solution. Wait
30-60 seconds, then rinse delicately with water.
5. Dry.
6. Examine the preparation under the microscope with the
objective for immersion.
STORE (±×¶÷¿°»ö Å°Æ®¿¡ ´ëÇÑ º¸°ü
¹æ¹ýÀÔ´Ï´Ù)
Store Gram Color Kit at 10-25¡ÆC in the original packaging. Keep away from
sources of heat and avoid excessive changes of temperature. In such conditions
the product Gram Color Kit will be valid until the expiry date shown on the
label. Do not use beyond that date. Eliminate without using if there are signs
of deterioration (changes in the color of the solutions or presence of
substantial precipitates).
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* This product may have contamination risk during the manufacturing or delivering, so please check before use it.