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Xylella fastidiosa (Xf) – ELISA Kit (DAS)

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      Xylella fastidiosa (Xf) – ELISA Kit (DAS)

       

       

      This ELISA product is a qualitative assay for the detection of Xylella fastidiosa (Xf), the causal agent of bacterial leaf scorch, Pierce's disease, and phony peach disease. This pathogen has a wide host range, including almond, blackberry, citrus, grapevine, and olive.

       

      The antibodies for this assay were screened against multiple isolates of three subspecies within the X. fastidiosa species, detecting all true positives, including those from infected Oleander. Exclusivity panels revealed no cross-reactivity with multiple common bacterial, fungal and viral pathogens. Moreover, no host-reaction was observed with healthy tissue from any common host of X. fastidiosa we tested.

       

      Xylella fastidiosa spreads throughout the plant via the xylem vessels and can be distributed differentially within the host. Due to this localization, we recommend testing petiole or stem samples from multiple locations on symptomatic plants. Please note that continuing external research suggests root tissue provides the most consistent accurate results when testing peach for the presence of X. fastidiosa.

       

      Pathoscreen Set Includes (Full kit) ;

      Antibody-coated 96-well microtiter plates

      Alkaline phosphatase enzyme conjugate

      RUB6, 1X Ready to use

      PNP substrate tablets, 5 mg each

      PNP substrate buffer, 5X concentrate

      Positive control

      PBST buffer, 20X concentrate, 50 mL

      TWEEN¢ç 20

      General extract buffer, GEB

      User Guide

       

      Pathoscreen Set Required but Not Included ;

      Buffer Instructions

      Logsheet

       

      Reagent Set Includes ;

      Capture antibody

      Alkaline phosphatase enzyme conjugate

      RUB6, 1X Ready to use

      96-well microtiter plates, solid* (*strip in single plate set)

      User Guide

       

      Reagent Set Required but Not Included ;

      Buffer Pack (ACC 00333)

      -       Carbonate coating buffer, 10X concentrate

      -       PNP substrate buffer, 5X concentrate

      -       PNP substrate tablets, 5 mg each

      -       PBST buffer, powder

      -       General extract buffer

      -       TWEEN¢ç 20

      Buffer Formulations from Chemicals

      Logsheet

       

       

      PACKAGE

       

      Description

      Quantity

      Format

      Cat. No

      PathoScreen¢ç Kit for Xf

      96 tests

      DAS ELISA

      AG-PSA34503/0096

      PathoScreen¢ç Kit for Xf

      288 tests

      DAS ELISA

      AG-PSA34503/0288

      PathoScreen¢ç Kit for Xf

      480 tests

      DAS ELISA

      AG-PSA34503/0480

      Reagent Set for Xf

      96 test

      DAS ELISA

      AG-SRA34503/0096

      Reagent Set for Xf

      500 tests

      DAS ELISA

      AG-SRA34503/0500

      Reagent Set for Xf

      1000 tests

      DAS ELISA

      AG-SRA34503/1000

      Reagent Set for Xf

      5000 tests

      DAS ELISA

      AG-SRA34503/5000

      Positive Control for Xf

       (ÇÊ¿ä ½Ã º°µµ±¸¸Å)

      Makes 2mL

       

      AG-LPC34503

      Negative Control for Xf, Grape leaves

       (ÇÊ¿ä ½Ã º°µµ±¸¸Å)

      Makes 2mL

       

      AG-LNC34503/GRAPE LF

      Negative Control for Xf, Peach leaves

       (ÇÊ¿ä ½Ã º°µµ±¸¸Å)

      Makes 2mL

       

      AG-LNC34503/PEACH LF

      Negative Control for Xf, Citrus stem

       (ÇÊ¿ä ½Ã º°µµ±¸¸Å)

      Makes 2mL

       

      AG-LNC34503/CITRUS STEM

       


       

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      * This product may have contamination risk during the manufacturing or delivering, so please check before use it.

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