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Xylella fastidiosa (Xf) – ELISA Kit (DAS)
This ELISA product is a qualitative assay for
the detection of Xylella fastidiosa (Xf), the causal agent of bacterial leaf
scorch, Pierce's disease, and phony peach disease. This pathogen has a wide
host range, including almond, blackberry, citrus, grapevine, and olive.
The antibodies for this assay were screened
against multiple isolates of three subspecies within the X. fastidiosa species,
detecting all true positives, including those from infected Oleander.
Exclusivity panels revealed no cross-reactivity with multiple common bacterial,
fungal and viral pathogens. Moreover, no host-reaction was observed with healthy
tissue from any common host of X. fastidiosa we tested.
Xylella fastidiosa spreads throughout the
plant via the xylem vessels and can be distributed differentially within the
host. Due to this localization, we recommend testing petiole or stem samples
from multiple locations on symptomatic plants. Please note that continuing
external research suggests root tissue provides the most consistent accurate
results when testing peach for the presence of X. fastidiosa.
Pathoscreen Set Includes (Full kit) ;
Antibody-coated 96-well microtiter plates
Alkaline phosphatase enzyme conjugate
RUB6, 1X Ready to use
PNP substrate tablets, 5 mg each
PNP substrate buffer, 5X concentrate
Positive control
PBST buffer, 20X concentrate, 50 mL
TWEEN¢ç 20
General extract buffer, GEB
User Guide
Pathoscreen Set Required but Not Included ;
Buffer Instructions
Logsheet
Reagent Set Includes ;
Capture antibody
Alkaline phosphatase enzyme conjugate
RUB6, 1X Ready to use
96-well microtiter plates, solid* (*strip in
single plate set)
User Guide
Reagent Set Required but Not Included ;
Buffer Pack (ACC 00333)
-
Carbonate coating buffer, 10X concentrate
-
PNP substrate buffer, 5X concentrate
-
PNP substrate tablets, 5 mg each
-
PBST buffer, powder
-
General extract buffer
-
TWEEN¢ç 20
Buffer Formulations from Chemicals
Logsheet
PACKAGE
|
Description |
Quantity |
Format |
Cat. No |
|
PathoScreen¢ç Kit for Xf |
96 tests |
DAS ELISA |
AG-PSA34503/0096 |
|
PathoScreen¢ç Kit for Xf |
288 tests |
DAS ELISA |
AG-PSA34503/0288 |
|
PathoScreen¢ç Kit for Xf |
480 tests |
DAS ELISA |
AG-PSA34503/0480 |
|
Reagent Set for Xf |
96 test |
DAS ELISA |
AG-SRA34503/0096 |
|
Reagent Set for Xf |
500 tests |
DAS ELISA |
AG-SRA34503/0500 |
|
Reagent Set for Xf |
1000 tests |
DAS ELISA |
AG-SRA34503/1000 |
|
Reagent Set for Xf |
5000 tests |
DAS ELISA |
AG-SRA34503/5000 |
|
Positive Control for Xf (ÇÊ¿ä ½Ã º°µµ±¸¸Å) |
Makes 2mL |
|
AG-LPC34503 |
|
Negative Control for Xf, Grape leaves (ÇÊ¿ä ½Ã º°µµ±¸¸Å) |
Makes 2mL |
|
AG-LNC34503/GRAPE LF |
|
Negative Control for Xf, Peach leaves (ÇÊ¿ä ½Ã º°µµ±¸¸Å) |
Makes 2mL |
|
AG-LNC34503/PEACH LF |
|
Negative Control for Xf, Citrus stem (ÇÊ¿ä ½Ã º°µµ±¸¸Å) |
Makes 2mL |
|
AG-LNC34503/CITRUS STEM |
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* This product may have contamination risk during the manufacturing or delivering, so please check before use it.













